Measurement
Part:BBa_K1913035
Designed by: Tianhe Wang Group: iGEM16_Wageningen_UR (2016-10-14)
Constitutive promoter with Guanine riboswitch BS-yxjA with mRFP
Measurement device includes a constitutive promoter, guanine riboswitche BS-yxjA and mRFP. The binding of these metabolites (guanine molecule) to the riboswitches on the 5’ UTR of the mRFP mRNA will attenuate translation of mRFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 814
Illegal AgeI site found at 926 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |